|
KMID : 0363819840180010055
|
|
Korean Journal of Nuclear Medicine
1984 Volume.18 No. 1 p.55 ~ p.62
|
|
|
^(125)I Labelling of Protein Using Immobilized Enzyme
|
|
|
|
|
Abstract
|
|
|
For an effective solid-phase labelling of protein with 125 I , studies on the immobilization of lactoperoxidase(LPO) on the inner wall of polystyrene tubes were carried out. Labelling of bovine serum albumin(BSA) and insulin was also practiced using the LPO immobilized tubes.
The immobilized enzyme of about 2. 5 pg/tube was sufficient for small scale labelling since the results of radio-paper chromatography of the labelling mixture of insulin indicated that the yields were sufficiently high(80%) even in the reactions conducted at room temperature for 60 sec. The results of the Sephadex column chromatography indicated that the labelled products were not contaminated with LP O-125 I , and the radiochemical purity of the products was more than 90%.
In considering the general trend that the 1251 labelled protein obtained by using LPO maintains its intactness better than those obtained by using chloramine-T, together with the tendency of yield enhancing with increase of reactants-concentration, the LPO immobilized tube method is estimated to be one of the simple methods of labelling. The product might be applicable without further purification.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|